SMART Platform

Mass Spectrometry and Proteomic Analysis Platform (SMART) – Université d’Artois

Welcome to SMART, a technical platform operated by the Laboratory of the Blood-Brain Barrier (LBHE) at the Université d’Artois.

Fundings* : 

Presentation: SMART is a core facility dedicated to molecular analysis. It is equipped with two mass spectrometers and two chromatographic systems for sample preparation. Our expertise covers a wide range of applications:

• Proteomics (large-scale identification and quantification of proteins)
• Analysis and quantification of small molecules
• Biomarker discovery
• Lipidomics
• Protein separation and quantification

Partnerships: As part of the LBHE, the SMART platform actively participates in the laboratory’s projects, while also collaborating with companies, academic laboratories and regional and national hospitals. Since 2006, we have developed numerous partnerships for both service provision and scientific collaboration. We are committed to providing high-quality services and adhere to strict confidentiality and responsiveness criteria. We offer transparent technical and financial packages supported by scientific expertise and access to state-of-the-art equipment.

Our activities: We support users of the SMART platform in carrying out custom scientific projects to address specific challenges. We offer two types of service:

• Service provision
• Scientific collaboration/partnership projects

Please do not hesitate to contact us directly for any enquiries regarding rates or additional information.

Services offered by SMART:

• Identification of proteins and peptides (in liquid or solid phase): proteins isolated by gel electrophoresis, peptide mixtures purified by nano- or micro-liquid chromatography, and various samples (e.g. animal/plant tissues, bacterial cultures, yeasts, cells, and biological fluids).
• Identification and quantification of small molecules: including drug quantification through our Blood-Brain Barrier model.
• Quantitative and differential proteomics: development of acquisition methods (SWATH-MS, DDA, DIA).
• Clinical proteomics approaches: adapted to biological samples (e.g. serum, plasma, urine, cerebrospinal fluid and tissues).
• Chemistry: quality control of synthetic molecules by mass spectrometry.

Lipidomics: analysis and quantification of lipids in biological samples using DDA and DIA SWATH-MS.

Our equipment :

Mass spectrometry:

• ZenoTOF 7600 + , Sciex.

• TripleTOF 5600 + , Sciex.

Other equipment :

• LC M-Class, Waters
• Ekspert nanoLC 425, Eksigent
• Selexion™ device for lipidomics
• Gel imaging and quantification analysis tools (SameSpots™)
• Gel electrophoresis devices (SDS-PAGE)
• Bioinformatics: advanced identification software MS-protein ProteinPilot; Analyst, MultiQuant, LipidView, and Sciex OS software

Contact us:

Sophie Duban-Deweer, Ph.D, Research Engineer - sophie.duban[at]univ-artois.fr

Johan Hachani, Study Engineer - johan.hachani[at]univ-artois.fr

Christophe Landry, Research Engineer - christophe.landry[at]univ-artois.fr

SMART platform phone: +33 3 21 79 17 28

*The ZenoTOF 7600+ project was co-funded by the European Union, the Hauts-de-France region, the CPER MOSOPS and the University of Artois.

The total funding amounted to 768 000€, with 632 000€ provided by the EU and 136 500€ by the Université d’Artois.